Production and purification of epsilon prototoxin produced by Clostridium perfringens type D
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چکیده
منابع مشابه
Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine [Estabilidade e toxicidade da protoxina epsilon de Clostridium perfringens tipo D]
Purified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of ...
متن کاملClostridium perfringens type D epsilon prototoxin. Some chemical, immunological and biological properties of a highly purified prototoxin.
WORTHINGTON, R. W., MOLDERS, MARIAS. G . & VAN RENSBURG, J . J. , 1973. Clostridium perfringens typeD epsilon prototoxin. Some chemical, immunological and biological properties of a highly purified preparation of proto toxin . Onderstepoort J. vet. Res. 40(4) 145152 (1973) Highly purified C/. perfringens typeD epsilon prototoxin was prepared by ammonium sulphate precipitation and DEAE cellulose...
متن کاملAquaporin-4 in acute cerebral edema produced by Clostridium perfringens type D epsilon toxin.
Sheep, particularly lambs, with high circulating levels of Clostridium perfringens type D epsilon toxin develop severe neurologic signs and often die suddenly. On microscopic examination, in the brain, there is microvascular endothelial injury and diffuse vasogenic edema. The aquaporin (AQP) family of membrane water-channel proteins, especially AQP-4, is important in the regulation of water bal...
متن کاملEpsilon-toxin plasmids of Clostridium perfringens type D are conjugative.
Isolates of Clostridium perfringens type D produce the potent epsilon-toxin (a CDC/U.S. Department of Agriculture overlap class B select agent) and are responsible for several economically significant enterotoxemias of domestic livestock. It is well established that the epsilon-toxin structural gene, etx, occurs on large plasmids. We show here that at least two of these plasmids are conjugative...
متن کاملIn silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B
Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective proteinexpression. Epsilon and Beta toxins are the most potent Clostridial toxins and cause disease in animals.This study describes in silico fusion of Clostridium perfringens types D and B epsilon and beta toxin genesthat was used for cloning in E.coli. The etx and cpb genes were...
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ژورنال
عنوان ژورنال: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
سال: 2002
ISSN: 0102-0935
DOI: 10.1590/s0102-09352002000300019